Primer Premier 6.25

Design and analyze the structures and properties of PCR primers by inputting data into the application to find the optimal combination of the PCR, multiplex and SNP genotyping primer characteristics. The program automatically calculates the most accurate melting temperature with the nearest thermodynamic algorithms.

Primer Premier is an application to design and analyze PCR primers. Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor thermodynamic algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in ranked order. Equipped with a handy calculator, you can easily manipulate sequences and analyze the results of your primer design.